Methods for Electrode Localization in Biological Tissue from 3D Ultrasound Data Martin Barva Abstrakt: Many surgical procedures consist of introducing a miniature surgical instrument such a needle, or electrode into biological tissue. The effectiveness of such procedures is enhanced, if the position of such instrument in tissue can be estimated in the course of intervention. We developed two methods for determining the position of a thin electrode located in biological tissue from 3D ultrasound data. The first method is based on the Parallel Integral Projection (PIP) transform. We experimentally verified that the axis of the electrode can be estimated from the maximum of PIP transformation. To accelerate the search for maximum, a hierarchical mesh-grid algorithm was implemented. The second method is based on model fitting. The electrode model is composed of a cubic polynomial approximating the electrode axis and the distribution of voxel intensities that was a priori estimated from acquired data. Model parameters are robustly estimated using the RANSAC estimator. Both methods were tested on two types of datasets: (i) simulated 3D datasets with known electrode position, (ii) data acquired with a 3D ultrasound system scanning a tissue mimicking phantom containing a tungsten electrode.